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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
29/12/2014 |
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Data da última atualização: |
07/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BENCKE-MALATO, M.; CABREIRA, C.; WIEBKE-STROHM, B.; BÜCKER-NETO, L.; MANCINI, E.; OSORIO, M. B.; HOMRICH, M. S.; TURCHETTO-ZOLET, A. C.; CARVALHO, M. C. C. G. de; STOLF, R.; WEBER, R. L. M.; WESTERGAARD, G.; CASTAGNARO, A. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; MARGIS-PINHEIRO, M.; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
MARTA BENCKE-MALATO, UFRGS; CAROLINE CABREIRA, UFRGS; BEATRIZ WIEBKE-STROHM, UFRGS; LAURO BÜCKER-NETO, UFRGS; ESTEFANIA MANCINI, Instituto de Agrobiotecnologia Rosario SA; MARINA B. OSORIO, UFRGS; MILENA S. HOMRICH, UFRGS; ANDREIA CARINA TURCHETTO-ZOLET, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSO - estagiária; RENATA STOLF, CNPSO - estagiària; RICARDO L. M. WEBER, UFRGS; GASTÓN WESTERGAARD, Instituto de Agrobiotecnologia Rosario SA; ATÍLIO P. CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres (EEAOC); RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARIA HELENA BODANESE-ZANETTINI, UFRGS. |
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Título: |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 14, n. 1, article 236, Sept. 2014. |
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Páginas: |
18 p. |
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ISSN: |
1471-2229 |
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DOI: |
10.1186/s12870-014-0236-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants. MenosBackground: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than ... Mostrar Tudo |
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Thesagro: |
Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114583/1/Genome-wide-annotation-of-the-soybean-WRKY-family-and-functional-characterization-of-genes-involved-in-response-to-Phakopsora-pachyrhizi-infection.pdf
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Marc: |
LEADER 02998naa a2200373 a 4500
001 2003866
005 2022-04-07
008 2014 bl uuuu u00u1 u #d
022 $a1471-2229
024 7 $a10.1186/s12870-014-0236-0$2DOI
100 1 $aBENCKE-MALATO, M.
245 $aGenome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection.$h[electronic resource]
260 $c2014
300 $a18 p.
520 $aBackground: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
650 $aSoybeans
650 $aSoja
700 1 $aCABREIRA, C.
700 1 $aWIEBKE-STROHM, B.
700 1 $aBÜCKER-NETO, L.
700 1 $aMANCINI, E.
700 1 $aOSORIO, M. B.
700 1 $aHOMRICH, M. S.
700 1 $aTURCHETTO-ZOLET, A. C.
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aSTOLF, R.
700 1 $aWEBER, R. L. M.
700 1 $aWESTERGAARD, G.
700 1 $aCASTAGNARO, A. P.
700 1 $aABDELNOOR, R. V.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tBMC Plant Biology$gv. 14, n. 1, article 236, Sept. 2014.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Cerrados. |
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Data corrente: |
25/02/2022 |
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Data da última atualização: |
25/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
KOTHARI, K.; BATTISTI, R.; BOOTE, K. J.; ARCHONTOULIS, S. V.; CONFALONE, A.; CONSTANTIN, J.; CUADRA, S. V.; DEBAEKE, P.; FAYE, B.; GRANT, B.; HOOGENBOOM, G.; JING, Q.; VAN DER LAAN, M.; SILVA, F. A. M. da; MARIN, F. R.; NEHBANDANI, A.; NENDEL, C.; PURCELL, L. C.; QIAN, B.; RUANE, A. C.; SCHOVING, C.; SILVA, E. H. F. M.; SMITH, W.; SOLTANI, A.; SRIVASTAVA, A.; VIEIRA JÚNIOR, N. A.; SLONE, S.; SALMERÓN, M. |
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Afiliação: |
KRITIKA KOTHARI, UNIVERSITY OF KENTUCKY; RAFAEL BATTISTI, UFG; KENNETH J. BOOTE, UNIVERSITY OF FLORIDA; SOTIRIOS V. ARCHONTOULIS, IOWA STATE UNIVERSITY; ADRIANA CONFALONE, UNIVERSIDAD NACIONAL DEL CENTRO DE LA PROVINCIA DE BUENOS AIRES; JULIE CONSTANTIN, UNIVERSITÉ DE TOULOUSE; SANTIAGO VIANNA CUADRA, CNPTIA; PHILIPPE DEBAEKE, UNIVERSITÉ DE TOULOUSE; BABACAR FAYE, INSTITUT DE RECHERCHE POUR LE D ́EVELOPPEMENT (IRD) ESPACE-DEV; BRIAN GRANT, AGRICULTURE AND AGRI-FOOD CANADA; GERRIT HOOGENBOOM, UNIVERSITY OF FLORIDA; QI JING, AGRICULTURE AND AGRI-FOOD CANADA; MICHAEL VAN DER LAAN, UNIVERSITY OF PRETORIA; FERNANDO ANTONIO MACENA DA SILVA, CPAC; FÁBIO RICARDO MARIN, ESALQ/USP; ALIREZA NEHBANDANI, GORGAN UNIVERSITY OF AGRICULTURAL SCIENCES AND NATURAL RESOURCE; CLAAS NENDEL, University of PotsdaM, Leibniz Centre for Agricultural Landscape ResearcH; LARRY C. PURCELL, UNIVERSITY OF ARKANSAS; BUDONG QIAN, AGRICULTURE AND AGRI-FOOD CANADA; ALEX C. RUANE, NASA GODDARD INSTITUTE FOR SPACE STUDIES; CÉLINE SCHOVING, UNIVERSITÉ DE TOULOUSE, TERRES INOVIA; EVANDRO H. F. M. SILVA, ESALQ/USP; WARD SMITH, AGRICULTURE AND AGRI-FOOD CANADA; AFSHIN SOLTANI, GORGAN UNIVERSITY OF AGRICULTURAL SCIENCES AND NATURAL RE-SOURCES; AMIT SRIVASTAVA, UNIVERSITY OF BONN; NILSON A. VIEIRA JÚNIOR, ESALQ/USP; STACEY SLONE, UNIVERSITY OF KENTUCKY; MONTSERRAT SALMERÓN, UNIVERSITY OF KENTUCKY. |
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Título: |
Are soybean models ready for climate change food impact assessments? |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
European Journal of Agronomy, v. 135, 126482, Apr. 2022. |
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DOI: |
https://doi.org/10.1016/j.eja.2022.126482 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract. An accurate estimation of crop yield under climate change scenarios is essential to quantify our ability to feed a growing population and develop agronomic adaptations to meet future food demand. A coordinated evaluation of yield simulations from process-based eco-physiological models for climate change impact assessment is still missing for soybean, the most widely grown grain legume and the main source of protein in our food chain. In this first soybean multi-model study, we used ten prominent models capable of simulating soybean yield under varying temperature and atmospheric CO2 concentration [CO2] to quantify the uncertainty in soybean yield simulations in response to these factors. Models were first parametrized with high quality measured data from five contrasting environments. We found considerable variability among models in simulated yield responses to increasing temperature and [CO2]. For example, under a + 3 °C temperature rise in our coolest location in Argentina, some models simulated that yield would reduce as much as 24%, while others simulated yield increases up to 29%. In our warmest location in Brazil, the models simulated a yield reduction ranging from a 38% decrease under + 3 °C temperature rise to no effect on yield. Similarly, when increasing [CO2] from 360 to 540 ppm, the models simulated a yield increase that ranged from 6% to 31%. Model calibration did not reduce variability across models but had an unexpected effect on modifying yield responses to temperature for some of the models. The high uncertainty in model responses indicates the limited applicability of individual models for climate change food projections. However, the ensemble mean of simulations across models was an effective tool to reduce the high uncertainty in soybean yield simulations associated with individual models and their parametrization. Ensemble, ensemble mean yield responses to temperature and [CO2] were similar to those reported from the literature. Our study is the first demonstration of the benefits achieved from using an ensemble of grain legume models for climate change food projections, and highlights that further soybean model development with experiments under elevated [CO2] and temperature is needed to reduce the uncertainty from the individual models. MenosAbstract. An accurate estimation of crop yield under climate change scenarios is essential to quantify our ability to feed a growing population and develop agronomic adaptations to meet future food demand. A coordinated evaluation of yield simulations from process-based eco-physiological models for climate change impact assessment is still missing for soybean, the most widely grown grain legume and the main source of protein in our food chain. In this first soybean multi-model study, we used ten prominent models capable of simulating soybean yield under varying temperature and atmospheric CO2 concentration [CO2] to quantify the uncertainty in soybean yield simulations in response to these factors. Models were first parametrized with high quality measured data from five contrasting environments. We found considerable variability among models in simulated yield responses to increasing temperature and [CO2]. For example, under a + 3 °C temperature rise in our coolest location in Argentina, some models simulated that yield would reduce as much as 24%, while others simulated yield increases up to 29%. In our warmest location in Brazil, the models simulated a yield reduction ranging from a 38% decrease under + 3 °C temperature rise to no effect on yield. Similarly, when increasing [CO2] from 360 to 540 ppm, the models simulated a yield increase that ranged from 6% to 31%. Model calibration did not reduce variability across models but had an unexpected effect on modifying yield res... Mostrar Tudo |
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Palavras-Chave: |
AgMIP; Agricultural Model Intercomparison and Improvement Project; Impacto das mudanças climáticas; Legume model; Model calibration; Model ensemble; Modelos de soja; Temperature Atmospheric CO2 concentration. |
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Thesagro: |
Glycine Max; Soja; Temperatura. |
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Thesaurus NAL: |
Models; Soybeans; Temperature. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232002/1/AP-Soybean-models-2022.pdf
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Marc: |
LEADER 04032naa a2200625 a 4500
001 2140426
005 2022-02-25
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.eja.2022.126482$2DOI
100 1 $aKOTHARI, K.
245 $aAre soybean models ready for climate change food impact assessments?$h[electronic resource]
260 $c2022
520 $aAbstract. An accurate estimation of crop yield under climate change scenarios is essential to quantify our ability to feed a growing population and develop agronomic adaptations to meet future food demand. A coordinated evaluation of yield simulations from process-based eco-physiological models for climate change impact assessment is still missing for soybean, the most widely grown grain legume and the main source of protein in our food chain. In this first soybean multi-model study, we used ten prominent models capable of simulating soybean yield under varying temperature and atmospheric CO2 concentration [CO2] to quantify the uncertainty in soybean yield simulations in response to these factors. Models were first parametrized with high quality measured data from five contrasting environments. We found considerable variability among models in simulated yield responses to increasing temperature and [CO2]. For example, under a + 3 °C temperature rise in our coolest location in Argentina, some models simulated that yield would reduce as much as 24%, while others simulated yield increases up to 29%. In our warmest location in Brazil, the models simulated a yield reduction ranging from a 38% decrease under + 3 °C temperature rise to no effect on yield. Similarly, when increasing [CO2] from 360 to 540 ppm, the models simulated a yield increase that ranged from 6% to 31%. Model calibration did not reduce variability across models but had an unexpected effect on modifying yield responses to temperature for some of the models. The high uncertainty in model responses indicates the limited applicability of individual models for climate change food projections. However, the ensemble mean of simulations across models was an effective tool to reduce the high uncertainty in soybean yield simulations associated with individual models and their parametrization. Ensemble, ensemble mean yield responses to temperature and [CO2] were similar to those reported from the literature. Our study is the first demonstration of the benefits achieved from using an ensemble of grain legume models for climate change food projections, and highlights that further soybean model development with experiments under elevated [CO2] and temperature is needed to reduce the uncertainty from the individual models.
650 $aModels
650 $aSoybeans
650 $aTemperature
650 $aGlycine Max
650 $aSoja
650 $aTemperatura
653 $aAgMIP
653 $aAgricultural Model Intercomparison and Improvement Project
653 $aImpacto das mudanças climáticas
653 $aLegume model
653 $aModel calibration
653 $aModel ensemble
653 $aModelos de soja
653 $aTemperature Atmospheric CO2 concentration
700 1 $aBATTISTI, R.
700 1 $aBOOTE, K. J.
700 1 $aARCHONTOULIS, S. V.
700 1 $aCONFALONE, A.
700 1 $aCONSTANTIN, J.
700 1 $aCUADRA, S. V.
700 1 $aDEBAEKE, P.
700 1 $aFAYE, B.
700 1 $aGRANT, B.
700 1 $aHOOGENBOOM, G.
700 1 $aJING, Q.
700 1 $aVAN DER LAAN, M.
700 1 $aSILVA, F. A. M. da
700 1 $aMARIN, F. R.
700 1 $aNEHBANDANI, A.
700 1 $aNENDEL, C.
700 1 $aPURCELL, L. C.
700 1 $aQIAN, B.
700 1 $aRUANE, A. C.
700 1 $aSCHOVING, C.
700 1 $aSILVA, E. H. F. M.
700 1 $aSMITH, W.
700 1 $aSOLTANI, A.
700 1 $aSRIVASTAVA, A.
700 1 $aVIEIRA JÚNIOR, N. A.
700 1 $aSLONE, S.
700 1 $aSALMERÓN, M.
773 $tEuropean Journal of Agronomy$gv. 135, 126482, Apr. 2022.
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